the present invention relates to a strain of bacillus subtilis selected from the group consisting of a) the strain deposited as dsm32324, b) the strain deposited as dsm32325 and c) a mutant strain of (a) or (b) that has sensitivity to ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol； and has inhibitory activity against e. coli and clostridium perfringens. the invention further relates to bacillus compositions comprising at least one strain of bacillus subtilis of the invention, preferably the strain of bacillus subtilis dsm32324 and / or the strain of bacillus subtilis dsm32325, as direct feed microbials (dfm), premixed , additive for animal feed or animal feed. the invention provides a method to improve one or more animal performance parameters selected from the group consisting of i) increased weight gain (wg), ii) lower feed conversion rate (fcr), iii) lower injury score due to necrotic enteritis, iv) lower frequency of necrotic enteritis, v) lower mortality from necrotic enteritis, vi) increase in the European production efficiency factor (epef) and vii) lower mortality, feeding a strain or composition according to the invention to an animal.a presente invenção refere-se a uma cepa de bacillus subtilis selecionada a partir do grupo que consiste em a) a cepa depositada como dsm32324, b) a cepa depositada como dsm32325 e c) uma cepa mutante de (a) ou (b) que tem sensibilidade à ampicilina, vancomicina, gentamicina, canamicina, estreptomicina, eritromicina, clindamicina, tetraciclina e cloranfenicol； e tem atividade inibidora contra e. coli e clostridium perfringens. a invenção refere-se ainda a composições de bacillus compreendendo ao menos uma cepa de bacillus subtilis da invenção, preferencialmente a cepa de bacillus subtilis dsm32324 e/ou a cepa de bacillus subtilis dsm32325, como microbianos de alimentação direta (dfm), pré-mistura, aditivo para ração animal ou ração animal. a invenção for
