The invention provides a bacterial strain selected from the group, including (a) ECA preserved as dsm32324, (b) ECA preserved as dsm32325, (c) mutant strains (a) or (b) that are sensitive to amphetamine, vancomycin, gentamicin, kanamicina, streptomycin, erythromycin and clindamicina, 4. Tetracycline cyanamide and ketamine, and have inhibitory effect on E.coli and Clostridium perpringens. The invention also relates to the composition of COD, including at least one subtropical cod strain, preferably dsm32324 subtropical cod strain and / or dsm32325 subtropical cod strain,As a microorganism directly used in food (DFM, "direct fed microbial"),Premezcla, animal feed additive or animal feed. The invention provides a method for improving one or more performance parameters of one or more selected animals, including (I) maximum weight increase (AP),（2） Low feed conversion (ICP),（3） The injury score of necrotizing enteritis is low, the incidence of necrotizing enteritis is low, the mortality of necrotizing enteritis is low, and the production efficiency is high in Europe,（7） The mortality rate is low, and the animals are fed with the invented strains or ingredients.La presente invención proporciona una cepa de Bacillus subtilis seleccionada del grupo que consiste en a) la cepa depositada como DSM32324, b) la cepa depositada como DSM32325, y c) una cepa mutante de (a) o (b) la cual tiene sensibilidad a la ampicilina, vancomicina, gentamicina, kanamicina, estreptomicina, eritromicina, clindamicina, tetraciclina y al cloranfenicol； y tiene actividad inhibidora contra E. coli y Clostridium perfringens. La invención se refiere además a composiciones de Bacillus que comprenden por lo menos una cepa de Bacillus subtilis de la invención, preferentemente la cepa de Bacillus subtilis DSM32324 y/o la cepa de Bacillus subtilis DSM32325, como producto microbiano para administración directa en el alimento (DFM, siglas en inglés correspondientes a “Direct Fed Microbial”), premezcla, aditivo
