An apparatus, system, and method for sampling a fluid from a tissue site is described. The system includes a collection fitting, a reduced-pressure source, and a specimen container. The collection fitting includes a tee-fitting having a first arm having a first union, a second arm having a second union, and a third arm. At least one lumen extends through the tee-fitting from the first arm to the third arm, and at least one lumen extends through the tee-fitting from the second arm to the third arm. A container union is coupled to the third arm. The reduced-pressure source is configured to be fluidly coupled to the first union, and the specimen container is configured to be coupled to the container union.