PROBLEM TO BE SOLVED: To provide a novel culture means capable of producing a heavier myocardial tissue and maintaining the tissue in a good survival state, thereby providing a myocardial tissue suitable for transplantation. A method for producing a multilayer myocardial tissue culture, which comprises culturing cardiomyocytes seeded on a fiber sheet made of a biocompatible polymer in a pseudo-microgravity environment. A multi-layered myocardial tissue culture that is profound and has a high survival rate obtained by the method. [Selection diagram] None【課題】より重厚な心筋組織を作製し、かつ該組織を良好な生存状態で維持し得る新規な培養手段を提供し、以て、移植に適した心筋組織を提供すること。【解決手段】生体適合性ポリマーからなるファイバーシート上に播種された心筋細胞を、擬微小重力環境下にて培養することを含む、多層心筋組織培養物の製造方法。該方法により得られる、重厚かつ生存率の高い多層心筋組織培養物。【選択図】なし
