The present invention provides uses of an Epilobium angustifolium extract in the manufacture of composition for inducing the gene expression of Transglutaminase, Keratin, Aquaporin and Filaggrin in keratinocytes, and promoting the keratinocyte secretion of hyaluronic acid, and effectively relieving the sensitive and reddening of the skin, and to effectively maintain the keratinocyte arrangement, the stratum corneum structure integrity, so as to enhance skin barrier function and make the skin produce more moisturizing factor. The Epilobium angustifolium extract is prepared by extracting Epilobium angustifolium using water, alcohols, or mixtures of water and alcohols as solvents.本發明提供一種柳蘭萃取物用於提升角質細胞中TGM、KRT、AQP及FLG基因表現量，以及促進角質細胞分泌玻尿酸並舒緩皮膚敏感、泛紅之醫藥組合物的用途，能有效維持角質細胞排列，使皮膚角質層結構完整，以提升皮膚屏障功能以及使皮膚形成更多保濕因子。其中該柳蘭萃取物係柳蘭以水、醇、或醇水混合物之溶劑所萃取而得。
