CHENG, HSUEH LING,郑雪玲,鄭雪玲,CHANG, CHI I,张志益,張誌益,CHEN, YO CHIA,陈又嘉,陳又嘉,CHOU, CHANG HUNG,周昌弘,周昌弘
申请号：
TW101149390
公开号：
TWI446913B
申请日:
2012.12.22
申请国别(地区):
TW
年份:
2014
代理人:
摘要：
An extract ofCucurbita moschatacontaining natural active substance for regulating blood glucose comprises (22E,24R)-24-methyl-6β-methoxy-5α-cholesta-7,22-diene-3β,5-diol and 3β-hydroxy-(22E,24R)-ergosta-5,88,22-trien-7-one. The extract ofCucurbita moschatais obtained by using a manufacture method comprising the following steps: extracting aCucurbita moschatasample with methanol to obtain a crude extract extracting the crude extract with ethyl acetate and water to obtain an ethyl acetate layer and a water layer fractionating the ethyl acetate layer with hexane and ethyl acetate as eluent to obtain fractions eluting by 70-80% ethyl acetate, and further evaporating and lyophilizing fractions to obtain the extract ofCucurbita moschata.一種南瓜萃取物,含有可調控血糖之天然活性成分,其包含(22E,24R)-24-甲基-6β-甲氧基-5α-膽甾醇-7,22-二烯-3β,5-二醇及(22E,24R)-3β-羥基麥角固醇-5,8,22-三烯-7-酮。上述南瓜萃取物係由以下方法製得:以甲醇對一南瓜樣品進行萃取,得一南瓜粗萃物;以乙酸乙酯與水對上述南瓜粗萃物進行萃取,得一乙酸乙酯層及一水層;以正己烷與乙酸乙酯混合液作為流動相,對固定於一矽膠管柱之上述乙酸乙酯層進行層析分離,收集以濃度為70~80%乙酸乙酯混合液所沖提之區分物,經減壓濃縮及冷凍乾燥後,以獲得上述南瓜萃取物。
