Engineering of rice varieties with enhanced resistances to both blast and bacterial blight diseases via CRISPR/Cas9

• 全文链接

通过CRISPR/Cas9工程化水稻品种，增强对稻瘟病和白叶枯病的抗性

关键词：

来源：

PLANT BIOTECHNOLOGY JOURNAL

全文链接：

//agri.nais.net.cn/topic/downloadFile/fca774b4-1009-4704-98e9-f26beeb4e387

来源地址：

//agri.nais.net.cn/topic/downloadFile/fca774b4-1009-4704-98e9-f26beeb4e387

资源所属：

杂交水稻专题

类型：

学术文献

语种：

英语

原文发布日期：

2021-12-04

摘要：

Rice blast and bacterial blight represent two of major diseases having devastating impact on the yield of rice in most rice-growing countries. Developments of resistant cultivars are the most economic and effective strategy to control these diseases. Here, we used CRISPR/Cas9-mediated gene editing to rapidly install mutations in three known broad-spectrum blast-resistant genes, Bsr-d1, Pi21 and ERF922, in an indica thermosensitive genic male sterile (TGMS) rice line Longke638S (LK638S). We obtained transgene-free homozygous single or triple mutants in T-1 generations. While all single and triple mutants showed increased resistance to rice blast compared with wild type, the erf922 mutants displayed the strongest blast resistance similar with triple mutants. Surprisingly, we found that Pi21 or ERF922 single mutants conferred enhanced resistance to most of tested bacterial blight. Both resistances in mutants were attribute to the up-regulation of SA- and JA-pathway associated genes. Moreover, phenotypic analysis of these single mutants in paddy fields revealed that there were no trade-offs between resistances and main agricultural traits. Together, our study provides a rapid and effective way to generate rice varieties with resistance to both rice blast and bacterial blight.