Different lengths, copies and expression levels of the mitochondrial atp6 gene in male sterile and fertile lines of carrot (Daucus carota L.)

• 全文链接

胡萝卜雄性不育系和可育系线粒体atp6基因的不同长度、拷贝数和表达水平

关键词：

来源：

Mitochondrial DNA Part A

全文链接：

//agri.nais.net.cn/topic/downloadFile/eec4ba94-215f-4b2a-a994-48e3ba55ab86

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资源所属：

种质资源保护与创制专题

类型：

学术文献

语种：

英语

原文发布日期：

2017-03-23

摘要：

The male-sterile carrot is an effective material for carrot breeding. The atp6 gene is involved in carrot fertility. However, the differences in lengths, copies, and expression profiles of the atp6 gene in fertile and male-sterile lines of carrot are unclear. In this study, one copy atp6 gene was found in the mtDNAs of ‘Kuroda’ (fertility, 954?bp) and ‘Wuye-BY’ (male sterility, 819?bp) carrot lines, while two copies atp6 genes (Wuye-L and Wuye-D, 954?bp and 819?bp, respectively) were found in the mtDNA of ‘Wuye’ (fertility). Two putative conserved domains have been detected in the carrot atp6 protein. Evolutionary analysis showed that the atp6 protein sequences of Wuye-L and Kuroda were clustered in the same branch, while Wuye-D and Wuye-BY were clustered in the same branch. The atp6 gene was higher expressed in the flowers of ‘Kuroda’ and ‘Wuye’ (Wuye-L), while lower expressed in ‘Wuye-BY’ and ‘Wuye’ (Wuye-D).