Effects of host-associated low-temperature probiotics in olive flounder (Paralichthys olivaceus) aquaculture

• 全文链接

宿主相关低温益生菌对牙鲆（Paralichthys olivaceus）养殖的影响

关键词：

来源：

Scientific Reports

全文链接：

//agri.nais.net.cn/topic/downloadFile/ee0d2367-917a-4cbc-9d87-dd70d71cc82b

来源地址：

https://www.nature.com/articles/s41598-024-52491-9

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2024-01-25

摘要：

This study investigated the effects of supplementation of low-temperature probiotics isolated from the intestines of olive flounder on the growth performance, digestibility, and regulation of intestinal microbiota and the expression of genes related to growth, immunity, and apoptosis in olive flounder. Bacteria showing high growth at approximately 15–20 °C, which is the temperature of olive flounder culture, were isolated and confirmed to be Pseudomonas species through 16S rRNA gene sequence analysis. Whole-genome sequencing revealed that the strain has a 6,195,122 bp single circular chromosome and a guanine–cytosine content of 59.9%. In the feeding trial, supplementation with 1 × 108 CFU/g of the isolate strain positively modulated growth performances, digestive enzyme activity, and gut microbiota composition of olive flounder. RT-qPCR for the comparison of growth, immunity, and apoptosis-related gene expression levels showed no significant differences between the groups. Therefore, the isolated host-associated low-temperature probiotics improved the growth performance of olive flounder by causing positive changes in digestive activity and intestinal microbial composition without affecting host gene expression.