Sensitive and specific detection of Listeria monocytogenes in food samples using imprinted upconversion fluorescence probe prepared by emulsion polymerization method

• 全文链接

乳液聚合制备的印迹上转换荧光探针对食品中单核增生李斯特菌的灵敏特异检测

关键词：

来源：

FOOD CHEMISTRY-X

全文链接：

//agri.nais.net.cn/topic/downloadFile/e84de3d7-d132-4158-b29f-9bbd3ec96bb9

来源地址：

https://www.sciencedirect.com/science/article/pii/S2590157524005066

资源所属：

农牧业信息化专题

类型：

学术文献

语种：

英语

原文发布日期：

2024-07-04

摘要：

Listeria monocytogenes (L. monocytogenes) is a foodborne pathogen with high morbidity and mortality rates, necessitating rapid detection methods. Current techniques, while reliable, are labor-intensive and not amenable to on-site testing. We report the design and synthesis of a novel imprinted upconversion fluorescence probe through Pickering emulsion polymerization for the specific detection of L. monocytogenes. The probe employs trimethylolpropane trimethacrylate and divinylbenzene as cross-linkers, acryloyl-modified chitosan as a functional monomer, and the bacterium itself as the template. The developed probe demonstrated high specificity and sensitivity in detecting L. monocytogenes, with a limit of detection of 72 CFU/mL. It effectively identified the pathogen in contaminated salmon and chicken samples, with minimal background interference. The integration of molecular imprinting and upconversion fluorescence materials presents a potent and reliable approach for the rapid and specific detection of L. monocytogenes, offering considerable potential for on-site food safety testing.