Expediting genome synthesis of Corynebacterium glutamicum with an artificial chromosome vector

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用人工染色体载体加速谷氨酸棒状杆菌的基因组合成

关键词：

来源：

Trends in Biotechnology

全文链接：

//agri.nais.net.cn/topic/downloadFile/e5ac8160-0081-46a0-8af3-56cf412e127c

来源地址：

https://www.sciencedirect.com/science/article/pii/S0167779925000824?via%3Dihub

资源所属：

马铃薯基因组设计育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-03-28

摘要：

Recent advances in genome synthesis have relied on scalable DNA assembly and delivery, and efficient recombination techniques. While these methods have enabled rapid progress for Escherichia coli and yeast, they are often inadequate for other microorganisms. Here, we devised a Corynebacterium glutamicum artificial chromosome (CAC), which combines a replicating system from a closely related strain with an innate partitioning system. This CAC vector can efficiently deliver DNA fragments up to 56 kb and maintain stability in C. glutamicum. Leveraging the CAC vector, we developed CAC Excision Enhanced Recombination (CACEXER), a streamlined strategy for iterative genome replacements in C. glutamicum. Using this approach, we integrated 361 kb (11%) of synthetic DNA into the genome, creating semi-synCG-A. This strain paves the way to establish C. glutamicum as the third industrial microorganism, alongside E. coli and Saccharomyces cerevisiae, to undergo large-scale genome synthesis.