Combinatorial High-Throughput Screening of Complex Polymeric Enzyme Immobilization Supports

• 全文链接

复合聚合酶固定化载体的组合高通量筛选

关键词：

来源：

Journal of the American Chemical Society

全文链接：

//agri.nais.net.cn/topic/downloadFile/dcf780cf-ec51-4740-a29f-d4e0df86fc25

来源地址：

https://pubs.acs.org/doi/10.1021/jacs.3c14273

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2024-03-19

摘要：

Recent advances have demonstrated the promise of complex multicomponent polymeric supports to enable supra-biological enzyme performance. However, the discovery of such supports has been limited by time-consuming, low-throughput synthesis and screening. Here, we describe a novel combinatorial and high-throughput platform that enables rapid screening of complex and heterogeneous copolymer brushes as enzyme immobilization supports, named combinatorial high-throughput enzyme support screening (CHESS). Using a 384-well plate format, we synthesized arrays of three-component polymer brushes in the microwells using photoactivated surface-initiated polymerization and immobilized enzymes in situ. The utility of CHESS to identify optimal immobilization supports under thermally and chemically denaturing conditions was demonstrated usingBacillus subtilisLipase A (LipA). The identification of supports with optimal compositions was validated by immobilizing LipA on polymer-brush-modified biocatalyst particles. We further demonstrated that CHESS could be used to predict the optimal composition of polymer brushes a priori for the previously unexplored enzyme, alkaline phosphatase (AlkP). Our findings demonstrate that CHESS represents a predictable and reliable platform for dramatically accelerating the search of chemical compositions for immobilization supports and further facilitates the discovery of biocompatible and stabilizing materials.