Spatial transcriptome and single-cell RNA sequencing reveal the molecular basis of cotton fiber initiation development

• 全文链接

空间转录组和单细胞RNA测序揭示了棉花纤维起始发育的分子基础

关键词：

来源：

PLANT JOURNAL

全文链接：

//agri.nais.net.cn/topic/downloadFile/dcb188c6-c585-4d03-89d0-182f55b63a5f

来源地址：

https://onlinelibrary.wiley.com/doi/10.1111/tpj.70064

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-03-14

摘要：

Recent advances in single-cell transcriptomics have greatly expanded our knowledge of plant development and cellular responses. However, analyzing fiber cell differentiation in plants, particularly in cotton, remains a complex challenge. A spatial transcriptomic map of ovule from -1 DPA, 0 DPA, and 1 DPA in cotton was successfully constructed, which helps to explain the important role of sucrose synthesis and lipid metabolism during early fiber development. Additionally, single-cell RNA sequencing (scRNA-seq) further highlighted the cellular heterogeneity and identified clusters of fiber developmental marker genes. Integration of spatial and scRNA-seq data unveiled key genes SVB and SVBL involved in fiber initiation, suggesting functional redundancy between them. These findings provide a detailed molecular landscape of cotton fiber development, offering valuable insights for enhancing lint yield.