Development of Multiple-Heading-Date mtl Haploid Inducer Lines in Rice

• 全文链接

水稻多抽穗期mtl单倍体诱导系的选育

关键词：

来源：

AGRICULTURE-BASEL

全文链接：

//agri.nais.net.cn/topic/downloadFile/d61e17d5-fcc2-42f3-b6c4-d1ffe8bee9cb

来源地址：

//agri.nais.net.cn/topic/downloadFile/d61e17d5-fcc2-42f3-b6c4-d1ffe8bee9cb

资源所属：

杂交水稻专题

类型：

学术文献

语种：

英语

原文发布日期：

2022-06-02

摘要：

In vivo doubled haploid (DH) production based on crossing heterozygous germplasm with mtl haploid inducer lines promises to transform modern rice (Oryza sativa) breeding. However, this technology is limited, as haploid inducers and pollen acceptors have asynchronous heading dates. To address this obstacle, we developed a panel of multiple-heading-date mtl haploid inducer lines that produce pollen for more than 35 days. We edited the MTL gene in a hybrid rice with the CRISPR-Cas9 system. We then selected transgene-free homozygous mutants in the T-1 generation and reproduced to T-4 generation by single-seed descent method. We obtained 547 mtl haploid inducers with diverse heading dates (from 73 to 110 days) and selected 16 lines comprising a core population with continuous flowering. The seed-setting rate and haploid induction rate (HIR) of the core panel were 4.0-12.7% and 2.8-12.0%, respectively. Thus, our strategy of using multiple-heading-date mtl haploid inducers could accelerate the use of in vivo DH technology in rice breeding.