Examining photosynthetic induction variation among historical cotton cultivars through time-integrated limitation analyses

• 全文链接

用时间积分极限分析法研究历史棉花品种光合诱导变异

关键词：

来源：

PLANT PHYSIOLOGY

全文链接：

//agri.nais.net.cn/topic/downloadFile/d057b5e8-6168-4e27-9851-45b9264f0954

来源地址：

https://academic.oup.com/plphys/article/199/1/kiaf157/8119349?login=true

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-09-01

摘要：

A thorough understanding of the biochemical, stomatal, and mesophyll components that limit photosynthetic induction is crucial for targeted improvement of crop productivity. However, compared with biochemical activation and stomatal conductance (gs), mesophyll conductance (gm) remains underexplored in induction studies. The fluorescence method (the variable J method) is a valid and widely accessible tool for gm measurement under steady-state conditions. Here, we experimentally validated the applicability of the fluorescence method under nonsteady-state conditions, demonstrating comparable induction kinetics of gm with the well-established carbon isotope method. Building on this validation, we combined the fluorescence method with gas-exchange measurements to comprehensively examine the induction kinetics of photosynthetic rate (A) and its associated components in a set of historical cotton (Gossypium hirsutum L.) cultivars. Our results showed no significant effect of the year of cultivar release on A during induction, suggesting that dynamic photosynthesis has not benefited from past selection efforts in cotton. Nonetheless, significant among-cultivar variations were observed in all measured induction traits, hinting at breeding opportunities for leveraging dynamic photosynthetic variation to boost crop productivity. Through induction-period-integrated limitation analysis, we further identified gs as the single most important limiter of photosynthetic induction across all cotton cultivars. Moreover, the analysis also demonstrated that accurately accounting for gm kinetics is essential for the unbiased acquisition of mechanistic insights into nonsteady-state photosynthetic physiology. We recommend that future induction studies incorporate gm measurements whenever possible to strengthen the knowledge base necessary for genetically enhancing dynamic carbon gain and crop yield in the field.Time-integrated limitation analyses incorporating mesophyll conductance reveal stomatal conductance as the primary limitation of photosynthetic induction among historical cotton cultivars.