Identification of candidate genes in cotton associated with specific seed traits and their initial functional characterization in Arabidopsis

• 全文链接

鉴定棉花中与特定种子性状相关的候选基因及其在拟南芥中的初步功能表征

关键词：

来源：

PLANT JOURNAL

全文链接：

//agri.nais.net.cn/topic/downloadFile/c716980c-a4d4-4bc0-a533-1618c883a747

来源地址：

https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.15982

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2022-11-01

摘要：

Oilseed crops are used to produce vegetable oil to satisfy the requirements of humans and livestock. Cotton (Gossypium spp.) is of great economic value because it is used as both an important textile commodity and a nutrient-rich resource. Cottonseed oil is rich in polyunsaturated fatty acids and does not contain trans fatty acids; hence, it is considered a healthy vegetable oil. However, research on the genetic basis for cottonseed protein content, oil production, and fatty acid composition is lacking. Here, we investigated the protein content, oil content, and fatty acid composition in terms of oleic acid (C18:1) and linoleic acid (C18:2) in mature cottonseeds from 318 Gossypium hirsutum accessions. Moreover, we examined the dynamic change of protein content and lipid composition including palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3) in developing seeds from 258 accessions at 10 and 20 days post-anthesis. Then, we conducted a genome-wide association study and identified 152 trait-associated loci and 64 candidate genes responsible for protein and oil-related contents in mature cottonseeds and ovules. Finally, six candidate genes were experimentally validated to be involved in the regulation of fatty acid biosynthesis through heterologous expression in Arabidopsis. These results comprise a solid foundation for expanding our understanding of lipid biosynthesis in cotton, which will help breeders manipulate protein and oil contents to make it a fully developed 'fiber, food, and oil crop'.