Promoting efficient synthesis and customization of sphingans based on metabolic engineering and synthetic biology strategies

• 全文链接

基于代谢工程与合成生物学策略促进鞘氨醇胶的高效合成与定制化

关键词：

来源：

Carbohydrate Polymers

全文链接：

//agri.nais.net.cn/topic/downloadFile/c4148713-cdee-4d4a-869c-80c83699076c

来源地址：

https://www.sciencedirect.com/science/article/pii/S014486172500517X?via%3Dihub

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2025-05-12

摘要：

Sphingans are important exopolysaccharides due to their unique functional characteristics and potential application prospects in various fields. In recent years, the chemical structure, biosynthesis and function of sphingans have been studied extensively. With the development of metabolic engineering and synthetic biology, problems that restricting the production capacity and the design of sphingans, such as complex synthetic path and unclear research background of the wildtype strain, would be expected to be solved to some extent. This review describes the structure and biosynthetic pathways of different sphingans, analyzes the feasibility of obtaining high-performance sphingans-producing strains via classical mutagenesis combined with high-throughput screening techniques and chassis cells construction, and focuses on discussing how to efficiently synthesize and customize sphingans based on metabolic engineering and synthetic biology strategies. These strategies include using highly effective tools like genomic metabolic network models (GSMM) and CRISPR to regulate metabolic pathways, as well as customizing sphingans with different molecular weight through molecular weight regulation and controllable substituent modification based on genetic engineering. At last, the main challenges and prospects are discussed.