New application of a dye-decolorizing peroxidase immobilized on magnetic nanoparticles for efficient simultaneous degradation of two mycotoxins

• 全文链接

磁性纳米颗粒固定化染料脱色过氧化物酶在高效同时降解两种真菌毒素中的新应用

关键词：

来源：

Food Chemistry

全文链接：

//agri.nais.net.cn/topic/downloadFile/c06a2512-a96e-48af-9094-ba0c768ea1c6

来源地址：

https://www.sciencedirect.com/science/article/pii/S0308814624029911?via%3Dihub

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2024-09-17

摘要：

Nowadays the enzymatic approaches are the most promising strategies for mycotoxins detoxification in food stuffs. Herein, the dye-decolorizing peroxidase RhDypB from Rhodococcus jostii was studied for its ability to degrade two mycotoxins in both free and the immobilized enzyme forms. This enzyme was recombinantly expressed and purified, while Fe3O4 nanoparticles were prepared and modified with chitosan as the immobilization carrier. The immobilized enzyme Fe3O4@CS@RhDypB demonstrated degradation rate of 85.61 % toward aflatoxin B1, while it was firstly found to be able to degrade zearalenone with the rate of 86.52 %, at pH 4.0 on 30 °C. The degradation products were identified as aflatoxin Q1 and 15-OH-ZEN respectively. After 5 cycles of reuse, Fe3O4@CS@RhDypB still exhibited degradation rates of 38.50 % and 49.76 % toward the mycotoxins, indicating its high reusability. Moreover, Fe3O4@CS@RhDypB exhibited excellent stability after 10 days of storage. This work identified potential applications of nanoparticle-immobilized enzyme for biodegradation of mycotoxins in food industry.