Foliar Application of Bacillus thuringiensis Enhances Tea Quality and Plant Defense via Phyllosphere Microbiome Modulation

• 全文链接

叶面喷施苏云金芽孢杆菌通过调控叶际微生物组提升茶叶品质与植株防御

关键词：

来源：

Agriculture-Basel 期刊

全文链接：

//agri.nais.net.cn/topic/downloadFile/c05a5b6f-8883-4f46-aaf6-739486c40770

来源地址：

https://www.mdpi.com/2077-0472/15/13/1386

资源所属：

茶学研究专题

类型：

学术文献

语种：

英语

原文发布日期：

2025-06-27

摘要：

The plant microbiome plays a crucial role in the health of the tea plant, while Bacillus thuringiensis (Bt) is widely utilized as a biological pesticide in tea gardens, promoting sustainable agricultural practices. However, the effects of Bt spraying on tea quality and the structure and function of the phyllosphere microbiome remain unclear. This study evaluated the effects of Bt spraying on tea quality, microbiome composition, diversity, and potential functions using tea leaf quality measurements and high-throughput sequencing of the 16S/ITS rDNA genes. Results showed that spraying Bt1 significantly increased the contents of free amino acids (by 15.27%), flavonoids (by 18.00%), soluble sugars (by 62.55%), and key compounds such as epicatechin gallate (by 10.50%), gallocatechin gallate (by 122.52%), and epigallocatechin gallate (by 61.29%), leading to improved leaf quality. Co-occurrence network analysis indicated that the community structure of both epiphytic and endophytic microbes became more complex after Bt treatment. The abundance of beneficial bacteria, such as Novosphingobium, Methylobacterium, and Sphingomonas, increased significantly, while pathogenic fungi like Aspergillus and Phyllosticta decreased. Functional prediction indicated enhanced amino acid metabolism, secondary metabolism, and carbohydrate metabolism, particularly the biosynthesis of flavonoids, which supports disease resistance and boosts secondary metabolite levels. Furthermore, Bt application reduced pathogenic fungi, enhancing the tea plant’s resistance to diseases. Overall, foliar spraying of Bt can positively alter the phyllosphere microbiome by enriching beneficial bacteria and improving metabolic functions, ultimately enhancing tea plant resistance and quality, and providing a scientific basis for sustainable pest management in tea cultivation.