Identification of three wheat near isogenic lines originated from CB037 on tissue culture and transformation capacities

• 全文链接

小麦CB037近等基因系的组织培养和转化能力鉴定

关键词：

来源：

Springer

全文链接：

//agri.nais.net.cn/topic/downloadFile/ad603be7-0a41-4824-80c6-c3dda88da144

来源地址：

https://link.springer.com/article/10.1007/s11240-022-02389-z

资源所属：

小麦遗传育种专题

类型：

学术文献

语种：

英语

原文发布日期：

2022-10-24

摘要：

To meet the increasing demand for wheat grains, it is essential to continuously improve wheat by commercial and advanced technologies. Wheat line CB037 has ideal agronomic traits, good bread quality, resistance to powdery mildew (PM), and high regeneration ability, and therefore it has been widely used in hybridization breeding and genetic transformation. Three near isogenic lines (NILs) from CB037 were identified in a previous study based on glutenin composition. In this study, the three NILs of CB037A, CB037B, and CB037C were investigated comparatively for agronomic traits, disease resistance to PM and stripe rust, and tissue culture and transformation efficiency. Results indicated that these NILs were highly resistant to PM, and CB037B was not only highly resistant to stripe rust but also had significantly higher regeneration ability than CB037A and CB037C in anther culture, immature and mature embryo culture. Particularly, positively transgenic wheat plants were obtained only from CB037B. Thereby CB037B carrying two types of translocations of 6AL·6V#2S and 1BL·1RS was identified to be a desirable material for wheat genetic transformation, which was of great significance for improving the transformation efficiency and accelerating the application of genetic engineering breeding technology in wheat.