Expanding the genome editing toolbox with designer CRISPR–Cas-like transposons

• 全文链接

利用设计CRISPR–Cas类转座子扩展基因组编辑工具箱

关键词：

来源：

NATURE METHODS

全文链接：

//agri.nais.net.cn/topic/downloadFile/ad03fdfd-14a6-4ab5-841f-d927cd044962

来源地址：

https://webofscience.clarivate.cn/wos/woscc/full-record/WOS:001318962800001

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2024-09-23

摘要：

Similarly to CRISPR-Cas systems, TnpB proteins from bacterial transposons can be employed as RNA-guided endonucleases for genome editing. By combining rational protein design and machine learning, ISDra2 TnpB variants with enhanced editing efficiency and a broader targeting range were developed, along with a prediction tool to design effective guiding RNAs.