Green recipe: Engineered yeast boosts D-lactic acid production

• 全文链接

绿色配方：工程酵母促进D -乳酸的产生

关键词：

来源：

EurekAlert

全文链接：

//agri.nais.net.cn/topic/downloadFile/acfc4a47-e230-4361-bcbe-b41f2434a01e

来源地址：

https://www.eurekalert.org/news-releases/1077550

资源所属：

饲料用酶工程

类型：

前沿资讯

语种：

英语

原文发布日期：

2025-03-20

摘要：

Seeking a more efficient way to produce D-lactic acid, the team turned to Komagataella phaffii, a yeast capable of utilizing methanol. Their goal was to pinpoint the optimal combination of D-lactate dehydrogenase (D-LDH) genes and promoters in K. phaffii that would maximize the yeast’s ability to produce D-lactic acid from methanol. D-LDH is a key enzyme responsible for converting precursor molecules into D-lactic acid, while promoters are DNA sequences that regulate gene expression. After testing five different D-LDH genes and eight promoters, the researchers identified an ideal mix that boosted D-lactic acid production by 1.5 times compared to other methanol-based methods. “To the best of our knowledge, our engineered yeast achieved the highest-ever reported yield using methanol as the sole carbon source,” Yamada said. These findings show that engineered yeast strains can be tailored to produce a wide range of useful compounds for commercial use. With growing global concerns over fossil fuel depletion and environmental impact, the ability to synthesize chemicals from renewable carbon sources like methanol is deemed a critical advancement for sustainability. “This study demonstrates that by carefully optimizing gene and promoter combinations, we can significantly enhance the efficiency of microbial processes, offering a viable alternative to traditional, petroleum-based chemical production,” Yamada said. The study was published in Biotechnology for Biofuels and Bioproducts.