Non-model bacteria as platforms for endogenous gene expression in synthetic biology

• 全文链接

非模型细菌作为合成生物学中内源基因表达的平台

关键词：

来源：

Nature Reviews Bioengineering

全文链接：

//agri.nais.net.cn/topic/downloadFile/a99b5c86-999c-472a-920c-0771adabfdda

来源地址：

https://www.nature.com/articles/s44222-025-00354-x

资源所属：

马铃薯基因组设计育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-09-24

摘要：

Microbial synthetic biology seeks to engineer bacterial genomes for industrial and biomedical applications, typically by applying heterologous gene expression in well-characterized model organisms, such as Escherichia coli. However, heterologous gene expression might cause metabolic disruptions, thereby impacting production efficiency and yield. In this Review, we highlight non-model organisms, such as Lacticaseibacillus and pseudomonads, for endogenous compound production, taking advantage of their evolutionary optimization for the production of certain metabolites and proteins. We first outline key limitations of heterologous production and then examine endogenous production pathways in non-model organisms for biotechnological and therapeutic applications. In particular, multi-omics approaches enable the discovery and characterization of these organisms, and phage-based genome refactoring enhances genome engineering capabilities. Finally, we outline key bottlenecks in the application of non-model organisms in biotechnology, including scale-up, costs and safety.