Pentyl leaf volatiles promote insect and pathogen resistance via enhancing ketol-mediated defense responses

• 全文链接

戊基叶挥发物通过增强缩酮介导的防御反应促进昆虫和病原体的抗性

关键词：

来源：

Plant Physiology 期刊

全文链接：

//agri.nais.net.cn/topic/downloadFile/a8e24ec6-b793-446f-87b1-140fffbfb629

来源地址：

https://academic.oup.com/plphys/article/197/2/kiae646/7920045?login=true

资源所属：

茶学研究专题

类型：

学术文献

语种：

英语

原文发布日期：

2024-12-10

摘要：

Plantsemit an array of volatile organic compounds in response to stresses. Six-carbongreen leaf volatiles (GLVs) and five-carbon pentyl leaf volatiles (PLVs) arefatty acid-derived compounds involved in intra- and inter-speciescommunications. Unlike extensively studied GLVs, the biological activities ofPLVs remain understudied. Maize (Zeamays L.) contains a unique monocot-specific lipoxygenase, ZmLOX6, that isunable to oxidize fatty acids and instead possesses a hydroperoxide lyase-likeactivity to specifically produce PLVs. Here, we show that disruption of ZmLOX6 reduced resistance to fall armyworm (FAW; Spodopterafrugiperda) and fungal pathogens Colletotrichumgraminicola and Cochliobolusheterostrophus. Metabolite profiling revealed that reduced resistance to insects andpathogens was associated with decreased production of PLVs and ketols,including the better studied α-ketol, 9,10-KODA (9-hydroxy-10-oxo-12(Z)-octadecadienoic acid). Exogenous PLV and 9,10-KODAtreatments rescued the resistance of lox6 mutants to FAW and the pathogens. Surprisingly, thesusceptible-to-herbivory lox6 mutants produced greater levels of wound-inducedjasmonates, suggesting potential substrate competition between JA and PLVpathway branches and highlighting a strong role of PLVs in defense againstinsects. Similarly, likely due to substrate competition between GLV and PLVsynthesis pathways, in response to C.graminicola infection, lox6 mutants accumulated elevated levels of GLVs, whichpromote susceptibility to this pathogen. Mutation of the GLV-producing ZmLOX10 in the lox6 mutant background reversed the susceptibility to C.graminicola, unveiling the contrasting roles of PLVs and GLVs in resistance to thispathogen. Overall, this study uncovered a potent signaling role of PLVs indefense against insect herbivory and fungal pathogens with distinct lifestyles.