SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication

• 全文链接

番茄TLFP8基因通过核内再复制调节气孔大小和密度，从而减少水分流失，提高水分利用效率

关键词：

来源：

Plant, Cell & Environment

全文链接：

//agri.nais.net.cn/topic/downloadFile/a23ed980-77e8-4054-b282-24c17f863cf7

来源地址：

https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13867

资源所属：

设施园艺专题

类型：

学术文献

语种：

英语

原文发布日期：

2020-08-16

摘要：

Improving plant water‐use efficiency (WUE) is important to plant survival and crop yield in the context of water limitation. In this study, SlTLFP8 (Tubby‐like F‐box protein 8) was identified as an osmotic‐induced gene in tomato. Transgenic tomato with up‐regulated expression of SlTLFP8 showed enhanced water‐deficient resistance, while knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit. SlTLFP8 overexpression significantly enhanced WUE by suppressing transpiration under both water‐sufficient and water‐deficient conditions. Further study showed that overexpressing SlTLFP8 significantly increased leaf epidermal cell size and thereby decreased stomatal density 10%–20%, conversely SlTLFP8 knockout resulted in decreased cell size and thereby increased stomatal density 20%–50%. SlTLFP8 overexpression and knockout modulated ploidy levels in leaf cells. Changes in expression of cell cycle related genes also indicated that SlTLFP8 affected cell size and stomatal density through endocycle transition. Despite changes in stomata density and transpiration, altering the expression of SlTLFP8 did not change photosynthesis. Additionally, biomass was not altered and there was little difference in fruit yield for transgenic and wild type lines under water‐sufficient and water‐deficient conditions. Our results demonstrate the effect of SlTLFP8 on endoreduplication and the potential of SlTLFP8 for improvement of WUE.