Gram-per-litre-scale production of lutein by engineered Corynebacterium

• 全文链接

利用工程化谷氨酸棒杆菌实现叶黄素克升级规模生产

关键词：

来源：

Nature Synthesis

全文链接：

//agri.nais.net.cn/topic/downloadFile/98116966-5234-4b3f-be20-197d0e8ca31b

来源地址：

https://www.nature.com/articles/s44160-025-00826-3

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2025-07-04

摘要：

Lutein is commonly used as a supplement for ocular health. However, commercial lutein is mainly extracted from marigold flowers, an inefficient process that includes secondary metabolites. Here, building on our previous research on lutein synthesis in engineered Escherichia coli, we report the development of a microbial platform for efficient lutein production using metabolically engineered Corynebacterium glutamicum. We engineered C. glutamicum to produce lycopene and introduced the lutein biosynthesis pathway. Then, we substantially enhanced lutein production by optimizing the haem pathway, engineering the P450 reductase and utilizing an optimal electron-channelling scaffold system to improve electron transfer reactions, addressing the rate-limiting steps in lutein synthesis. This approach achieved a substantial increase in lutein production, reaching a concentration of 1.78 g l−1, with a content of 19.51 mg per gram dry cell weight and a productivity of 32.88 mg l−1 h−1 in fed-batch fermentation. This approach allows gram-per-litre-scale microbial production of lutein.