Engineering lipase TLL to improve its acid tolerance and its biosynthesis in Trichoderma reesei for biodiesel production from acidified oil

• 全文链接

改造脂肪酶TLL，提高其耐酸能力并改善其在里氏木霉中的生物合成，以实现酸化油制备生物柴油

关键词：

来源：

Bioresource Technology

全文链接：

//agri.nais.net.cn/topic/downloadFile/813ab7b6-7f0e-413d-b280-01cad4dcbc26

来源地址：

https://www.sciencedirect.com/science/article/pii/S0960852424012252?via%3Dihub

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2024-09-24

摘要：

Lipases catalyze the synthesis of biodiesel, which is an important renewable alternative energy source. Cost-efficient conversion of waste acidified oil to biodiesel entails acid-tolerant lipases which have not been extensively studied. This study showed that the commonly used Thermomyces lanuginosus lipase TLL displayed a weak acid tolerance and an unsatisfactory performance in biodiesel production from acidified oil. Directed evolution of TLL identified one TLL-T3 variant with three residue substitutions (A69S/V150P/N222G). TLL-T3 displayed significantly enhanced acid tolerance, and its application in acidified oil treatment led to a biodiesel yield up to 90 % (w/w). A scaled-up production of TLL-T3 in Trichoderma reesei was further achieved and the highest extracellular lipase activity reached 16,123 U/mL after fermentation optimization. These results provide new insights into structural adaptation to acid tolerance by lipases and show that TLL-T3 holds great potential in commercial biodiesel production from waste acidified oil.