Metabolic mechanism, responses, and functions of genes HDH1, HDH3, and GST1 of tea ( Camellia sinensis L.) to the insecticide thiamethoxam
茶树HDH1、HDH3和GST1基因对杀虫剂噻虫嗪的代谢机制、反应和功能
- 关键词：
- 来源：
- Journal of Hazardous Materials 期刊
- 类型：
- 学术文献
- 语种：
- 英语
- 原文发布日期：
- 2024-12-25
- 摘要：
- Misuse of insecticides such as thiamethoxam (TMX) not only affects the quality of tea but also leaves residues in tea. Therefore, exploring the metabolic mechanisms of TMX in tea plants can evaluate effects of pesticides on the environment and human health. Here, effects of TMX on tea plants were studied. Malondialdehyde (MDA) content reached a maximum of 12.59 nmol/g fresh weight (FW) on 1st d under X (the recommended dose: 0.015 kg a.i./ha) of TMX. Under 2 X (0.03 kg a.i./ha), the catalase, glutathione S-transferase and superoxide dismutase activity were increased by 45.0 %, 55.5 %, and 49.7 % at 7 d respectively. Metabolomic and transcriptomic analyses revealed that TMX significantly affected amino acid metabolism, flavonoid biosynthesis and glutathione metabolism, and induced the expression of 3-hydroxyisobutyric acid dehydrogenase genes (CsHDH1 and CsHDH3) and glutathione S-transferase gene (CsGST1). The three genes were transiently expressed in Nicotiana benthamiana for the first time to verify the function of TMX degradation, with the degradation rate of 59.2 %-85.3 % at X. This study elucidated the response of tea plants to abiotic stress on the molecular-scale perspective, and the molecular approaches could serve as a model for the study on pesticide metabolism in plants.
- 所属专题：
- 60
