A single-nucleotide mutation of G301A in GaIAA14 confers leaf curling in Gossypium arboreum

• 全文链接

GaIAA14中G301A的单核苷酸突变导致棉花叶片卷曲

关键词：

来源：

FRONTIERS IN PLANT SCIENCE

全文链接：

//agri.nais.net.cn/topic/downloadFile/6806bac5-fcdf-46da-8afa-4036a4f965cd

来源地址：

https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1645239/full

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-07-22

摘要：

Cotton is a crucial fiber and oil crop, playing a significant role in the textile and food industries. Its yield heavily relies on photosynthesis, a process that primarily occurs in the leaves. Consequently, leaf morphology stands as a vital agronomic trait in cotton breeding. However, research on the molecular mechanisms underlying cotton leaf morphogenesis remains relatively limited. Here we identified a curly leaf mutant (CU) in Gossypium arboreum by ethyl methylsulfonate (EMS) mutagenesis. The genetic analysis revealed that the curly leaf trait in this mutant is a semi-dominant characteristic controlled by a single gene. The map-based cloning of the CU locus showed a single-nucleotide mutation from G to A at the 301st positions in AUX/IAA14 protein (GaIAA14), which resulted in an amino acid substitution from valine (V) to isoleucine (I). After silencing GaIAA14 through virus-induced gene silencing (VIGS) technology in CU mutant, the leaves exhibited a flattened phenotype, indicating that GaIAA14 is a key gene regulating leaf curling in cotton. Comparative transcriptomic RNA-Seq analysis revealed significant changes in the expression levels of most auxin-related genes, suggesting that the mutation disrupts auxin signaling transduction. These findings establish a foundation for further functional studies of this gene and provide research strategies for leaf morphology improvement.