Exploring genetic diversity of wild and related tetraploid wheat species Triticum turgidum and Triticum timopheevii
野生及近缘四倍体小麦Triticum turgidum和Triticum timopheevii的遗传多样性研究
- 关键词：
- 来源：
- ScienceDirect
- 类型：
- 学术文献
- 语种：
- 英语
- 原文发布日期：
- 2023-06-01
- 摘要：
- The domestication bottleneck has reduced genetic diversity in wheat, necessitating the use of wild relatives in breeding programs. Wild tetraploid wheat are widely used in the breeding programs but with morphological characters, it is difficult to distinguish these, resulting in misclassification/mislabeling or duplication of accessions in the Gene bank.The study aims to explore Genotyping by sequencing (GBS) to characterize wild and domesticated tetraploid wheat accessions to generate a core set of accessions to be used in the breeding program. TASSEL-GBS pipeline was used for SNP discovery, fastStructure was used to determine the population structure and PowerCore was used to generate a core sets. Nucleotide diversity matrices of Nie’s and F-statistics (FST) index were used to determine the center of genetic diversity. We found 65 % and 47 % duplicated accessions in Triticum timopheevii and T. turgidum respectively. Genome-wide nucleotide diversity and FST scan uncovered a lower intra and higher inter-species differentiation. Distinct FST regions were identified in genomic regions belonging to domestication genes: non-brittle rachis (Btr1) and vernalization (VRN-1). Our results suggest that Israel, Jordan, Syria, and Lebanon as the hub of genetic diversity of wild emmer; Turkey, and Georgia for T. durum; and Iraq, Azerbaijan, and Armenia for the T. timopheevii. Identified core set accessions preserved more than 93 % of the available genetic diversity. Genome wide association study (GWAS) indicated the potential chromosomal segment for resistance to leaf rust in T. timopheevii.
- 所属专题：
- 68
