MYB regulation of GST/GT mediates red petal spot development in cotton

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MYB调控GST/GT介导棉花红花瓣斑点发育

关键词：

来源：

CROP JOURNAL

全文链接：

//agri.nais.net.cn/topic/downloadFile/646f7c75-1317-4218-a039-c92bb7bb7611

来源地址：

https://www.sciencedirect.com/science/article/pii/S2214514125000972?via%3Dihub

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-06-01

摘要：

Red petal spots are beneficial for attracting cotton pollinators and producing hybrid seeds, and the anthocyanin pathway is generally regarded as a metabolic cause of petal coloration. The current study identified an MYB-encoding gene (Gar07G09390, GaMYB) as a candidate gene involved in cotton coloration by map-based cloning, and this MYB could positively regulate a candidate glutathione S transferase gene (Gar07G08900, GaGST). To unveil potentially involved genes within the GaMYB-regulating-GaGST route, color metabolites of both GaMYB- and GaGST-virus-induced gene silencing (VIGS) petals were investigated, revealing that they were largely glycosyl-decorated flavonoids. Accordingly, a transcriptomic survey of both VIGS petals identified a glycosyl-transferase gene (GaGT, Gar02G15390). Notably, this GaGT is adjacent to one of the genome-wide association study loci concerning petal spots in Gossypium arboreum, and it is also positively regulated by GaMYB. This new regulatory route including both GST and GT regulated by MYB is conserved among the three cotton species examined in this study (Gossypium arboreum, Gossypium hirsutum, and Gossypium barbadense). Accordingly, comprehensively evaluating the influence of these candidates and their homologs on cotton coloration may provide a more in-depth understanding of cotton coloration, ultimately facilitating the breeding of more colorful cotton.