Molecular traits of MAPK kinases and the regulatory mechanism of GhMAPKK5 alleviating drought/salt stress in cotton

• 全文链接

棉花MAPK激酶的分子特性及GhMAPKK5缓解干旱/盐胁迫的调控机制

关键词：

来源：

PLANT PHYSIOLOGY

全文链接：

//agri.nais.net.cn/topic/downloadFile/5e8f85d9-87f0-4aac-88a4-585feb9007be

来源地址：

https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiae415/7733478?login=true

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2024-09-04

摘要：

Mitogen-activated protein kinase kinases (MAPKKs) play a critical role in the mitogen-activated protein kinase (MAPK) signaling pathway, transducing external stimuli into intracellular responses and enabling plant adaptation to environmental challenges. Most research has focused on the model plant Arabidopsis (Arabidopsis thaliana). The systematic analysis and characterization of MAPKK genes across different plant species, particularly in cotton (Gossypium hirsutum), are somewhat limited. Here, we identified MAPKK family members from 66 different species, which clustered into five different sub-groups, and MAPKKs from four cotton species clustered together. Through further bioinformatic and expression analyses, GhMAPKK5 was identified as the most responsive MAPKK member to salt and drought stress among the 23 MAPKKs identified in Gossypium hirsutum. Silencing GhMAPKK5 in cotton through virus-induced gene silencing (VIGS) led to quicker wilting under salt and drought conditions, while overexpressing GhMAPKK5 in Arabidopsis enhanced root growth and seed germination under these stresses, demonstrating GhMAPKK5's positive role in stress tolerance. Transcriptomics and Yeast-Two-Hybrid assays revealed a MAPK cascade signal module comprising GhMEKK (mitogen-activated protein kinase kinase kinases)3/8/31-GhMAPKK5-GhMAPK11/23. This signaling cascade may play a role in managing drought and salt stress by regulating transcription factor genes, such as WRKYs, which are involved in the biosynthesis and transport pathways of ABA, proline, and RALF. This study is highly important for further understanding the regulatory mechanism of MAPKK in cotton, contributing to its stress tolerance and offering potential in targets for genetic enhancement.Genome-wide identification and transcriptomic analyses indicate that a mitogen-activated protein kinase kinase signaling cascade plays a role in managing drought and salt stress in cotton.