Biodegradation Characteristics and Mechanism of Aflatoxin B1 by Bacillus amyloliquefaciens from Enzymatic and Multiomics Perspectives

• 全文链接

解淀粉芽孢杆菌对黄曲霉毒素B1的降解特性及机理

关键词：

来源：

Journal of Agricultural and Food Chemistry

全文链接：

//agri.nais.net.cn/topic/downloadFile/588aabf6-787d-4e24-a12b-f5d73dfb713a

来源地址：

https://pubs.acs.org/doi/10.1021/acs.jafc.4c04055

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2024-07-03

摘要：

Aflatoxin B1 (AFB1), a mycotoxin and natural carcinogen, commonly contaminates cereals and animal feeds, posing serious health risks to human and animal. In this study, Bacillus amyloliquefaciens ZG08 isolated from kimchi could effectively remove 80.93% of AFB1 within 72 h at 37 °C and pH 7.0. Metabolome and transcriptome analysis showed that metabolic processes including glycerophospholipid metabolism and amino acid metabolism were most affected in B. amyloliquefaciens ZG08 exposed to AFB1. The adaptation mechanism likely involved activation of the thioredoxin system to restore intracellular redox equilibrium. The key genes, tpx and gldA, overexpressed in Escherichia coli BL21, achieved degradation rates of 60.15% and 47.16% for 100 μg/kg AFB1 under optimal conditions of 37 °C and pH 8.0 and 45 °C and pH 7.0, respectively. The degradation products, identified as AFD1, were less cytotoxic than AFB1 in HepG2 cells. These findings suggest potential strategies for utilizing probiotics and engineered enzymes in AFB1 detoxification.