Plant growth promotion via priming with volatile organic compounds emitted from Bacillus vallismortis strain EXTN-1
利用死亡谷芽孢杆菌EXTN-1释放的挥发性有机物诱导植物生长
- 关键词：
- 来源：
- Frontiers in Microbiology 期刊
- 类型：
- 学术文献
- 语种：
- 英语
- 原文发布日期：
- 2025-01-10
- 摘要：
- Volatileorganic compounds (VOCs) produced by potential plant growth-promotingrhizobacteria (PGPR) play an important role in plant interactions. However, themechanisms underlying this phenomenon are not well understood. Our findingsshow that the influence of VOCs from the PGPR strain Bacillus vallismortis (EXTN-1) on tobacco plant growth is dependent on the culture media used. TheVOCs released from sugar-rich media such as potato dextrose agar (PDA) andKing’s B (KB) media were highly effective. However, exposure to VOCs fromnutrient agar (NA), tryptic soy agar (TSA), and Luria-Bertani (LB) resulted inchlorosis and stunted plant growth. This effect was caused by the discharge ofa large amount of ammonia that altered the pH of the plant growth media. Seedlingsexposed to VOCs for 10 days exhibited improved growth even after the VOCs wereeliminated under greenhouse conditions. Priming of seeds with VOCs for 24 and48 h induced higher growth than the untreated control, and seeds with 48 hexposure were better as compared to 24 h treatment. Chemical characterizationof VOCs emitted by EXTN-1 in different media using solid-phase microextraction(SPME) coupled with gas chromatography-mass spectrometry (GC-MS) showed thepresence of 2,3-butanedione and monoxime in all spectra. However, 1-butanol wasthe prominent peak in VOC of EXTN-1 grown in KB and NA, while acetoin washighest in PDA, followed by KB. Heneicosane and benzaldehyde were exclusivelyproduced in NA media, and these synthetic compounds improved growth in theI-plate assay. This work indicates that VOCs released from EXTN-1 are importantfor the growth-promoting effect of EXTN-1.
- 所属专题：
- 60
