Protein Engineering of Substrate Specificity toward Nitrilases: Strategies and Challenges

• 全文链接

针对腈酶底物特异性的蛋白质工程：策略和挑战

关键词：

来源：

Journal of Agricultural and Food Chemistry

全文链接：

//agri.nais.net.cn/topic/downloadFile/4d9025a8-1d2c-4a13-8f1c-b6acd4a0b928

来源地址：

https://pubs.acs.org/doi/10.1021/acs.jafc.4c09599

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2025-01-10

摘要：

Nitrilase is extensively applied across diverse sectors owing to its unique catalytic properties. Nevertheless, in industrial production, nitrilases often face issues such as low catalytic efficiency, limited substrate range, suboptimal selectivity, and side reaction products, which have garnered heightened attention. With the widespread recognition that the structure of enzymes has a direct impact on their catalytic properties, an increasing number of researchers are beginning to optimize the functional characteristics of nitrilases by modifying their structures, in order to meet specific industrial or biotechnology application needs. Particularly in the artificial intelligence era, the innovative application of computer-aided design in enzyme engineering offers remarkable opportunities to tailor nitrilases for the widespread production of high-value products. In this discussion, we will briefly examine the structural mechanism of nitrilase. An overview of the protein engineering strategies of substrate preference, regioselectivity and stereoselectivity are explored combined with some representative examples recently in terms of the substrate specificity of enzyme. The future research trends in this field are also prospected.