Single-cell transcriptome reveals dominant subgenome expression and transcriptional response to heat stress in Chinese cabbage
利用单细胞转录组测序，揭示大白菜显性亚基因组表达和对热胁迫的转录反应
- 关键词：
- 来源：
- Genome Biology
- 类型：
- 学术文献
- 语种：
- 英语
- 原文发布日期：
- 2022-12-19
- 摘要：
- Chinese cabbage (Brassica rapa ssp. pekinensis) experienced a whole-genome triplication event and thus has three subgenomes: least fractioned, medium fractioned, and most fractioned subgenome. Environmental changes affect leaf development, which in turn influence the yield. To improve the yield and resistance to different climate scenarios, a comprehensive understanding of leaf development is required including insights into the full diversity of cell types and transcriptional net-works underlying their specificity.Here, we generate the transcriptional landscape of Chinese cabbage leaf at single-cell resolution by performing single-cell RNA sequencing of 30,000 individual cells. We characterize seven major cell types with 19 transcriptionally distinct cell clus-ters based on the expression of the reported marker genes. We find that genes in the least fractioned subgenome are predominantly expressed compared with those in the medium and most fractioned subgenomes in different cell types. Moreover, we gener-ate a single-cell transcriptional map of leaves in response to high temperature. We find that heat stress not only affects gene expression in a cell type-specific manner but also impacts subgenome dominance.Our study highlights the transcriptional networks in different cell types and provides a better understanding of transcriptional regulation during leaf develop-ment and transcriptional response to heat stress in Chinese cabbage.
- 所属专题：
- 59
