Creation of Two-Line Fragrant Glutinous Hybrid Rice by Editing the Wx and OsBADH2 Genes via the CRISPR/Cas9 System

• 全文链接

通过CRISPR/Cas9系统编辑Wx和OsBADH2基因创建两系香糯杂交稻

关键词：

来源：

International Journal of Molecular Sciences

全文链接：

//agri.nais.net.cn/topic/downloadFile/44910c32-8629-4ce6-a3f4-8ad85d35e03f

来源地址：

//agri.nais.net.cn/topic/downloadFile/44910c32-8629-4ce6-a3f4-8ad85d35e03f

资源所属：

杂交水稻专题

类型：

学术文献

语种：

英语

原文发布日期：

2023-01-03

摘要：

Global food security has benefited from the development and promotion of the two-line hybrid rice system. Excellent eating quality determines the market competitiveness of hybrid rice varieties based on achieving the fundamental requirements of high yield and good adaptability. Developing sterile and restorer lines with improved quality for two-line hybrid breeding by editing quality genes with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 is an efficient and practical alternative to the lengthy and laborious process of conventional breeding to improve rice quality. We edited Wx and OsBADH2 using CRISPR/Cas9 technology to produce both homozygous male sterile mutant lines and homozygous restorer mutant lines with Cas9-free. These mutants have a much lower amylose content while having a significantly higher 2-acetyl-1-pyrroline aroma content. Based on this, a fragrant glutinous hybrid rice was developed without too much effect on most agronomic traits. This study demonstrates the use of CRISPR/Cas9 in creating two-line fragrant glutinous hybrid rice by editing the components of the male sterile and the restorative lines.