Cotton GhBRC1 regulates branching, flowering, and growth by integrating multiple hormone pathways
棉花GhBRC1通过整合多种激素途径调控分枝、开花和生长
- 关键词：
- 来源：
- CROP JOURNAL
- 类型：
- 学术文献
- 语种：
- 英语
- 原文发布日期：
- 2022-02-01
- 摘要：
- Cotton architecture is partly determined by shoot branching and flowering patterns. GhBRC1 was previously identified by RNA-seq analysis of nulliplex-branching and normal-branching cotton. However, the roles of GhBRC1 in cotton remain unclear. In the present study, investigations of nuclear localization and transcriptional activity indicated that GhBRC1 has characteristics typical of transcription factors. Gene expression analysis showed that GhBRC1 was highly expressed in axillary buds but displayed different expression patterns between the two branching types. Overexpression of GhBRC1 in Arabidopsis significantly inhibited the number of branches and promoted flowering. In contrast, silencing GhBRC1 in cotton significantly promoted seedling growth. GhBRC1 was induced by multiple hormones, including strigolactones, which promoted seedling growth and seed germination of Arabidopsis plants overexpressing GhBRC1. Consistent with these findings, RNA-seq analysis of virus-induced gene silencing treated cotton revealed that a large number of genes were differentially expressed between GhBRC1-silenced and control plants, and these genes were significantly enriched in plant hormone signalling pathways. Together, our data indicates that GhBRCI regulates plant branching and flowering through multiple regulatory pathways, especially those regulating plant hormones, with functions partly differing from those of Arabidopsis BRC1. These results provide insights into the molecular mechanisms controlling plant architecture, which is important for breeding cotton with ideal plant architecture and high yield. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
- 所属专题：
- 171
