A dominant negative mutation of GhMYB25-like alters cotton fiber initiation, reducing lint and fuzz

• 全文链接

GhMYB25类显性负性突变改变棉纤维起始，减少皮棉和绒毛

关键词：

来源：

PLANT CELL

全文链接：

//agri.nais.net.cn/topic/downloadFile/3355044a-69c1-409f-8a12-1f0183c741dc

来源地址：

https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koae068/7623567?login=true

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2024-03-06

摘要：

Cotton (Gossypium hirsutum) fibers, vital natural textile materials, are single-cell trichomes that differentiate from the ovule epidermis. These fibers are categorized as lint (longer fibers useful for spinning) or fuzz (shorter, less useful fibers). Currently, developing cotton varieties with high lint yield but without fuzz remains challenging due to our limited knowledge of the molecular mechanisms underlying fiber initiation. This study presents the identification and characterization of a naturally occurring dominant negative mutation GhMYB25-like_AthapT, which results in a reduced lint and fuzzless phenotype. The GhMYB25-like_AthapT protein exerts its dominant negative effect by suppressing the activity of GhMYB25-like during lint and fuzz initiation. Intriguingly, the negative effect of GhMYB25-like_AthapT could be alleviated by high expression levels of GhMYB25-like. We also uncovered the role of GhMYB25-like in regulating the expression of key genes such as GhPDF2 (PROTODERMAL FACTOR 2), CYCD3; 1 (CYCLIN D3; 1), and PLD (Phospholipase D), establishing its significance as a pivotal transcription factor in fiber initiation. We identified other genes within this regulatory network, expanding our understanding of the determinants of fiber cell fate. These findings offer valuable insights for cotton breeding and contribute to our fundamental understanding of fiber development.A dominant negative mutation of GhMYB25-like inhibits fuzz initiation but only slightly reduces lint initiation by compensating with higher wild-type protein level during lint cell differentiation.