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Bacterial spore surface display system for enzyme stabilization in food industry: Principles, applications and efficiency optimization strategies

面向食品工业酶稳定的细菌芽孢表面展示技术:原理、应用及效率优化

关键词:
来源:
Trends in Food Science & Technology
来源地址:
https://doi.org/10.1016/j.tifs.2025.105276
类型:
学术文献
语种:
英语
原文发布日期:
2025-09-02
摘要:
Enzymes serve as green biological catalysts with irreplaceable roles in the food industry. However, free enzymes face several challenges, including structural instability, irreversible activity loss, and high production costs, which restrict their industrial implementation. While current enzyme immobilization technologies using magnetic nanoparticles, liposomes, and metal-organic frameworks show promise, they encounter critical bottlenecks such as mass transfer limitations, enzyme activity loss, and high preparation costs. Bacterial spore surface display systems (BSSDS) utilizing bacterial spores as carriers have emerged as a promising enzyme immobilization platform, offering enhanced stability. This review comprehensively analyzes sporulation and spore resistance mechanisms while systematically elucidating fundamental BSSDS principles. Both recombinant and non-recombinant system construction principles are examined, followed by a thorough assessment of current BSSDS applications in the food industry, including food safety, wastewater treatment, and food processing. Various optimization strategies for enhancing display efficiency are critically evaluated, and emerging trends in the field are discussed to provide insights for industrial-scale implementation. The recombinant system provides superior long-term stability and cost-effectiveness through reusability, which positions it as a promising candidate for the food industry. Through systematic optimization strategies, advancements have been achieved in display efficiency. These technological innovations establish BSSDS as an increasingly promising platform for industrial enzyme immobilization, delivering sustainable and cost-efficient biocatalytic solutions to the food industry. Future development efforts should focus on multi-enzyme synergistic display systems, intelligent responsive carriers for controllable enzyme regulation, and exploring novel anchoring proteins to further expand the scope and scale of displayable enzymes across food industry applications.
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