Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans

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MORC-1的调控是秀丽隐杆线虫CSR-1介导的种系基因许可机制的关键

关键词：

来源：

Science Advances

全文链接：

//agri.nais.net.cn/topic/downloadFile/29521a8c-90d3-4c72-b7a7-e0a733394802

来源地址：

https://www.science.org/doi/10.1126/sciadv.ado4170

资源所属：

马铃薯基因组设计育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-06-20

摘要：

The Argonaute CSR-1 is essential for germline development in C. elegans. Loss of CSR-1 leads to the down-regulation of thousands of germline-expressed genes, supporting a model in which CSR-1 “licenses” gene expression via a poorly understood mechanism. In contrast, a small subset of genes is up-regulated in csr-1 mutants, including morc-1, which encodes a conserved GHKL-type ATPase. We show that morc-1 is overexpressed in csr-1 mutants and accumulates over CSR-1 licensed targets, coinciding with aberrant gain of H3K9me3, reduced H3K36me3, and transcriptional repression. Notably, loss of morc-1 fully rescues these chromatin defects and partially restores gene expression and fertility in csr-1 mutants. Conversely, ectopic overexpression of MORC-1 in the wild-type germ line is sufficient to repress CSR-1 licensed targets and severely compromise fertility. These findings support a model in which CSR-1 prevents MORC-1 overexpression and consequent misregulation of CSR-1 licensed genes.