CRISPR–Cas applications in agriculture and plant research

• 全文链接

CRISPR-Cas 在农业和植物研究中的应用

关键词：

来源：

Nature Reviews Molecular Cell Biology

全文链接：

//agri.nais.net.cn/topic/downloadFile/27a6df2b-d3f7-4cfc-aa77-00829c1ffd27

来源地址：

https://www.nature.com/articles/s41580-025-00834-3

资源所属：

马铃薯基因组设计育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-03-07

摘要：

Growing world population and deteriorating climate conditions necessitate the development of new crops with high yields and resilience. CRISPR–Cas-mediated genome engineering presents unparalleled opportunities to engineer crop varieties cheaper, easier and faster than ever. In this Review, we discuss how the CRISPR–Cas toolbox has rapidly expanded from Cas9 and Cas12 to include different Cas orthologues and engineered variants. We present various CRISPR–Cas-based methods, including base editing and prime editing, which are used for precise genome, epigenome and transcriptome engineering, and methods used to deliver the genome editors into plants, such as bacterial-mediated and viral-mediated transformation. We then discuss how promoter editing and chromosome engineering are used in crop breeding for trait engineering and fixation, and important applications of CRISPR–Cas in crop improvement, such as de novo domestication and enhancing tolerance to abiotic stresses. We conclude with discussing future prospects of plant genome engineering.