Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli

• 全文链接

利用代谢工程大肠杆菌生产含硫氨基酸的微生物

关键词：

来源：

BIOTECHNOLOGY ADVANCES

全文链接：

//agri.nais.net.cn/topic/downloadFile/2364f80c-093d-481a-9457-3f3824d9a5d8

来源地址：

https://doi.org/10.1016/j.biotechadv.2024.108353

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2024-04-11

摘要：

L-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in costeffective media, its robustness for industrial processes, the well-characterized metabolism, and the availability of molecular tools for genetic engineering. This review focuses on the genetic and molecular mechanisms involved in the production of these sulfur-containing amino acids in E. coli. Additionally, we systematically summarize the metabolic engineering strategies employed to enhance their production, including the identification of new targets, modulation of metabolic fluxes, modification of transport systems, dynamic regulation strategies, and optimization of fermentation conditions. The strategies and design principles discussed in this review hold the potential to facilitate the development of strain and process engineering for direct fermentation of sulfur-containing amino acids.