QTL Mapping and Candidate Gene Analysis for Cotton Fiber Quality and Early Maturity Using F2 and F3 Generations

• 全文链接

棉花纤维品质与早熟的F2、F3代QTL定位及候选基因分析

关键词：

来源：

PLANTS-BASEL

全文链接：

//agri.nais.net.cn/topic/downloadFile/09b08ac5-6363-4331-b078-2fb09add149f

来源地址：

https://www.mdpi.com/2223-7747/14/7/1063

资源所属：

棉花遗传育种

类型：

学术文献

语种：

英语

原文发布日期：

2025-03-29

摘要：

Cotton is the most important natural fiber-producing crop globally. High-quality fiber and early maturity are equally important breeding goals in the cotton industry. However, it remains challenging to synchronously improve these traits through conventional breeding techniques. To identify additional genetic information relating to fiber quality and early maturity, 11 phenotypic traits for the F2 and F3 generations were tested, and quantitative trait loci (QTL) mapping was performed. Candidate genes were analyzed using published RNA-seq datasets and qRT-PCR assays. All 11 tested traits showed bi-directional transgressive segregation, and most traits followed an approximately normal distribution. Overall, significant positive and significant negative correlations were observed among these traits. During cotton breeding, varieties with strong boll-setting ability can be selected from early-maturing materials that have high-quality fiber. A total of 102 QTLs were mapped, including 4 major and 3 stable QTLs. qFL-D13-1 was mapped in both the F2 and F3 generations, achieving a 3.94% to 11.39% contribution rate to the phenotypic variation. Three genes located in the QTL regions were identified based on their high expression levels in the three evaluated RNA-seq datasets. Ghir_A04G014830.1, covered by qHNFFB-A4-1 and qFU-A4-1, encoded ACLA-1. Ghir_D13G015010.1, encoding VTC2, and Ghir_D13G016670.1, encoding GA2OX1, were in the stable QTL qFL-D13-1 region. The qRT-PCR results suggested that these three genes may be involved in regulating seed development, fiber initiation, and fiber elongation. Overall, these findings contribute additional information for the breeding of high-yield, high fiber quality, and early-maturity varieties, as well as serve as a foundation for research on the underlying molecular mechanisms.