PROBLEM TO BE SOLVED: To provide a method of inducing differentiation of pluripotent stem cells, such as induced pluripotent stem cells (hereinafter abbreviated as iPS cells), to hepatocytes in a short period of time, and a substance to be used in the method.SOLUTION: This invention relates to development of a novel differentiation inducing culture medium (HDI) for inducing differentiation of iPS cells into hepatoblasts. This invention relates to a method for inducing differentiation of iPS cells into hepatoblasts using HDI in which oncostatin M, FPH1, M50054, NEAA, sodium pyruvate, nicotinamide, and L-glutamine are added to conventional hepatocyte selection medium (HSM).SELECTED DRAWING: NoneCOPYRIGHT: (C)2016,JPO&INPIT【課題】多能性幹細胞、例えば人工多能性幹細胞(iPS細胞)から肝細胞を短期間で分化誘導する方法、並びに該方法に使用する物質の提供。【解決手段】iPS細胞から肝芽細胞を分化誘導するための新規分化誘導培養培地(HDI)を開発。該培地は従来の肝細胞選択培地(HSM)に対してオンコスタチンM、FPH1、M50054、NEAA、ピルビン酸ナトリウム、ニコチンアミド、及びL-グルタミンが添加されているHDIを用いてiPS細胞から肝芽細胞を分化誘導する方法。【選択図】なし
