Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
Verfaillie, Catherine M.,Reyes, Morayma,Furcht, Leo T.
申请号:
AU2011203281
公开号:
AU2011203281B2
申请日:
2011.06.30
申请国别(地区):
AU
年份:
2012
代理人:
摘要:
#$%^&*AU2011203281B220121213.pdf#####MULTIPOTENT ADULT STEM CELLS, SOURCES THEREOF, METHODS OF OBTAINING AND MAINTAINING SAME, METHODS OF DIFFERENTIATION THEREOF, METHODS OF USE THEREOF AND CELLS DERIVED THEREOF Abstract Methods and compositions are provided for circularizing target sequences in a sample. In particular, ligation oligonucleotides are employed to selectively hybridize with the target such that the target can be ligated into a closed circular target. Rolling circle amplification can then be performed directly on the target sequence for subsequent detection and analysis.