Boer, Viktor Marius,Broers, Nicolette Jasmijn,Lawrence, Adam G.
申请号：
AU2014273054
公开号：
AU2014273054A1
申请日:
2014.06.02
申请国别(地区):
AU
年份:
2015
代理人:
摘要：
The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity a polypeptide having ent-Kaurene synthase activity a polypeptide having ent-Kaurene oxidase activity and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.
