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Method of manufacturing a membrane protein
专利权人:
独立行政法人理化学研究所
发明人:
横山 茂之,下野 和実,白水 美香子,五島 美絵
申请号:
JP2009540056
公开号:
JP5704677B2
申请日:
2008.11.05
申请国别(地区):
JP
年份:
2015
代理人:
摘要:
A method is provided for producing a membrane protein folded to its native structure or active structure in a lipid disc or a liposome. The method comprises the following steps: (a) preparing a reaction solution for cell-free protein synthesis containing a polynucleotide encoding a membrane protein, a steroidal detergent, and a phospholipid, wherein the steroidal detergent is contained at a concentration higher than its critical micelle concentration; (b) decreasing the concentration of said steroidal detergent in the reaction solution; and (c) synthesizing the membrane protein simultaneously with formation of a lipid disk or liposome into which the synthesized membrane protein is integrated.
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