REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
发明人:
HONG, YEON GYU,JUNG, MYEONG GEUN,KIM, SUN CHEOL,LEE, BONG CHUN,LEE, DONG CHANG,SONG, SEOK BO
申请号：
KR20020079311
公开号：
KR20040051385(A)
申请日:
2002.12.12
申请国别(地区):
韩国
年份:
2004
代理人:
摘要：
PURPOSE: Provided is an isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens), which is released from culture extract of isolate BWC98-105(Sclerotinia sp.), thereby withering the subterranean stem and stem of the white clover continuously, and inhibiting the generation thereof consequently to reduce the consumption of agricultural chemicals. CONSTITUTION: An isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens) by using BWC98-105(Sclerotinia sp.) comprises the steps of: culturing BWC98-105 strain in PD broth medium for 7 days, followed by systematic extraction with an organic solvent; fractioning the extraction with butanol and ethylacetate; separating the butanol and ethylacetate fractions using the column filled with reverse phase silica gel(C18); and analyzing the separated fractions by HPLC, wherein a second metabolic product having herbicidal effect on the white clover(Trifolium repens) is pr
