As for this invention, human hair papillar cell and hair follicle kerachinosaito (the cell which forms the entity of the hair follicle. The destination of the signal from the hair papilla) it mixes, it designates that human hair follicle structure is reconstructed with in vivo as purpose. Culture manner of the hair papillar cell where this invention features that the hair papillar cell is cultured under existing of the FGF material, the BMP-2/BMP-4 material and the WNT material, maintains the hair induction potency is offered.本発明は、ヒト毛乳頭細胞と毛嚢ケラチノサイト(毛嚢の主体をなす細胞。毛乳頭からのシグナルの受け手)を混合し、ヒト毛嚢構造をin vivoで再構成することを目的とする。本発明は、FGF系材料、BMP-2/BMP-4系材料及びWNT系材料の存在下で毛乳頭細胞を培養することを特徴とする、毛誘導能を維持した毛乳頭細胞の培養方法を提供する。